DispersinB(R) Overproduction Using a Novel Approach Published in the Journal of Industrial Microbiology & Biotechnology
WINNIPEG, MANITOBA -- (MARKET WIRE) -- 07/21/09 -- Kane Biotech Inc. (TSX VENTURE: KNE), a biotechnology company engaged in the development of products that prevent and disperse microbial biofilms is pleased to announce that some of its ongoing internal research on the Company's patented DispersinB® antibiofilm technology has been published in the latest online edition of the Journal of Industrial Microbiology & Biotechnology (DOI 10.1007/s10295-009-0613-0; Impact Factor: 2.0).
The publication, entitled "Enhanced expression of engineered ACA-less Beta-1,6-N-acetylglucosanimidase (dispersin B) in Escherichia coli" authored by Dr. Nandadeva Yakandawala et al, demonstrates the effectiveness of ACA-less mRNA and the advantage of GFP (Green Fluorescent Protein) tagging for enhanced DispersinB® production and quantification.
"This publication demonstrates the feasibility of commercial scale DispersinB® production using a common E. coli expression system," stated Gord Froehlich, President and CEO of Kane Biotech. "As we move towards commercialization of our technology, it is important to confirm the feasibility of cost effectively producing DispersinB®," added Mr. Froehlich.
"This study shows the versatility of the GFP tagging technique in the optimization of fermentation conditions for improving the production of DispersinB® and other proteins of commercial importance in E. coli.," stated Dr. Sri Madhyastha, VP-Research & CSO of Kane Biotech. "Furthermore, a seven-fold increase in DispersinB® production in a 30 L fermentor with minimal optimization of fermentation conditions reported in this publication shows the potential of E. coli strain overexpressing ACA-less dspB gene for commercial scale production of DispersinB® under optimized fermentation conditions."
"This is Kane's second DispersinB® antibiofilm enzyme related research publication, which adds to the growing list of publications on DispersinB® from research labs all over the world," added Dr. Madhyastha.
About Kane Biotech Inc.
Kane Biotech is a biotechnology company engaged in the development of products to prevent and disperse biofilms. Biofilms develop when bacteria and other microorganisms form a protective matrix that acts as a shield against attack. When in a biofilm, bacteria become highly resistant to antibiotics, biocides, disinfectants, high temperatures and host immune responses. This resiliency contributes to human health problems such as recurrent urinary tract infections, medical device associated infections and tooth decay.
Kane Biotech uses patent protected technologies based on molecular mechanisms of biofilm formation/dispersal and methods for finding compounds that inhibit or disrupt biofilms. The Company has evidence that these technologies have potential to significantly improve the ability to prevent and/or destroy biofilms in several medical and industrial applications.
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Contacts: Kane Biotech Inc. Gord Froehlich President & Chief Executive Officer 204-477-7592 204-453-1314 (FAX) info@kanebiotech.com www.kanebiotech.com
Released July 21, 2009